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KMID : 1134820180470080780
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Journal of the Korean Society of Food Science and Nutrition
2018 Volume.47 No. 8 p.780 ~ p.785
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Effects of High Hydrostatic Pressure and Ultrafiltration Treatment on Physiological Activities of Germinated Black Soybeans
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Kim Min-Young
Lee Yoon-Jeong
Song Myeong-Seob
Oh Hyun-Ah
Jang Gwi-Yeong
Kim Kyung-Mi
Lee Jun-Soo
Jeong Heon-Sang
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Abstract
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This study was performed to investigate the effects of high hydrostatic pressure (HHP) treatment and ultrafiltration on the antioxidant and angiotensin converting enzyme (ACE) inhibitory activities of protein extracts in germinated black soybeans. Black soybeans were germinated and subjected to HHP, followed by preparation of extracts and fractions with molecular weight. The highest 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) (3.58 mg ascorbic acid equivalents (AAE)/g) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) (2.02 mg Trolox equivalents (TE) /g) radical scavenging activities and ACE inhibitory activities (51.35%) of protein extracts were observed at 150 MPa treatment after germination for 4 days. ABTS and DPPH radical scavenging activities of fractions were higher in PM2 (MW 3∼<10 kDa) with 6.52 mg AAE/g and 2.95 mg TE/g as compared to protein extracts. The strong ACE inhibitory effect (74.37%) of the fraction was observed at PM2 fraction at a concentration of 2 mg/mL. These results suggest that low molecular peptides derived from black soybeans subjected to HHP after germination may mediate physiological activity.
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KEYWORD
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black soybean, protein extract, germination, high hydrostatic pressure treatment, ultrafiltration
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